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GPRC6A Rabbit pAb

Anti-GPRC6A Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222129

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S222129
Product Name:   Anti-GPRC6A Rabbit Polyclonal Antibody
Synonyms:   GPCR; bA86F4.3
UNIPROT ID:   Q5T6X5 (Gene Accession – NP_683766 )
Background:    Members of family C of the G protein-coupled receptor (GPCR) superfamily, such as GPRC6A, are characterized by an evolutionarily conserved amino acid-sensing motif linked to an intramembranous 7-transmembrane loop region. Several members of GPCR family C, including GPRC6A, also have a long N-terminal domain (summary by Pi et al., 2005 [PubMed 16199532]).
Immunogen:    Synthetic peptide of human GPRC6A
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 20-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 222129(GPRC6A Antibody) at a dilution of 1/20(Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 222129(Anti-GPRC6A Antibody) at dilution 1/20.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela and 293T cell lysates;
Primary antibody: 222129(GPRC6A Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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