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GRP78 BiP (9E4) Mouse mAb

Anti-GRP78 BiP (9E4) Mouse Monoclonal Antibody
5/5

$349.00

Cat.#:  N261401

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N261401
Product Name:   Anti-GRP78 BiP (9E4) Mouse Monoclonal Antibody
Synonyms:   HSPA5; GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP
UNIPROT ID:   P11021
Background:    When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as ‘immunoglobulin heavy chain-binding protein’ (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).
Immunogen:    Purified recombinant human BiP/GRP78 (C-terminus) protein fragments expressed in E.coli.
Applications:    WB,IHC-F,IHC-P,ICC/IF
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    9E4-2A7-H6
MW:    Calculated MW: 72 kDa; Observed MW: 78 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Tags & Cell Markers
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of GRP78 BiP (9E4) in HeLa using BiP/GRP78 (Cterminus) antibody.

Western blot analysis of BiP/GRP78 (Cterminus) in Hela, C6, Lncap and MDA-MB-468 lysates using BiP/GRP78 (Cterminus) antibody.

Immunohistochemistry analysis of paraffin-embedded Colorectal cancer using BiP/GRP78 (Cterminus) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
          Publications