Email: info@neweastbio.com   |  Call: 6109452007

GSDMA Rabbit pAb

Anti-GSDMA Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219464

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219464
Product Name:   Anti-GSDMA Rabbit Polyclonal Antibody
Synonyms:   GSDM; FKSG9; GSDM1
UNIPROT ID:   Q96QA5 (Gene Accession – BC109197 )
Background:    May promote pyroptosis (Probable). Upon cleavage in vitro of genetically engineered GSDMA, the released N-terminal moiety binds to some types of lipids, such as possibly phosphatidylinositol (4,5)-bisphosphate. Homooligomerizes within the membrane and forms pores of 10 -15 nanometers (nm) of inner diameter, triggering cell death. Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown (Probable).
Immunogen:    Fusion protein of human GSDMA
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 150-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 219464(GSDMA Antibody) at a dilution of 1/150(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the fusion protein and then with 219464(Anti-GSDMA Antibody) at dilution 1/150.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: 293T, Hela and LO2 cell lysates;
Primary antibody: 219464(GSDMA Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
          Publications