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H2AC4 Rabbit pAb

Anti-H2AC4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220599

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220599
Product Name:   Anti-H2AC4 Rabbit Polyclonal Antibody
Synonyms:   H2A/m; H2AFM; HIST1H2AB
UNIPROT ID:   P04908 (Gene Accession – NP_003504 )
Background:    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Immunogen:    Synthetic peptide of human H2AC4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 220599(H2AC4 Antibody) at a dilution of 1/30(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 220599(Anti-H2AC4 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 220599(Anti-H2AC4 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with synthetic peptide and then with D261744(Anti-H2AC4 Antibody) at dilution 1/30.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Hela cells, 231 cells, K562 cells, human fetal brain tissue;
Primary antibody: 220599(H2AC4 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 minutes
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