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H2AJ Rabbit pAb

Anti-H2AJ Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219198

   Size:   100 μl

In Stock

            Product Description          
Cat.#:   S219198
Product Name:   Anti-H2AJ Rabbit Polyclonal Antibody
Synonyms:   H2AFJ
UNIPROT ID:   Q9BTM1 (Gene Accession – BC003602 )
Background:    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is located on chromosome 12 and encodes a replication-independent histone that is a variant H2A histone. The protein is divergent at the C-terminus compared to the consensus H2A histone family member. This gene also encodes an antimicrobial peptide with antibacterial and antifungal activity.[provided by RefSeq, Oct 2015]
Immunogen:    Fusion protein of human H2AJ
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 200-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 219198(H2AJ Antibody) at a dilution of 1/170(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 219198(Anti-H2AJ Antibody) at dilution 1/170.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 219198(Anti-H2AJ Antibody) at a dilution of 1/170.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D226037(Anti-H2AJ Antibody) at dilution 1/170.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Jurkat cell lysate;
Primary antibody: 219198(H2AJ Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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