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H4C1 Rabbit pAb

Anti-H4C1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219437

   Size:   100 μl

In Stock

            Product Description          
Cat.#:   S219437
Product Name:   Anti-H4C1 Rabbit Polyclonal Antibody
Synonyms:   H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4FA; H4-16; H4C11; H4C12; H4C13; H4C14; H4C15; HIST1H4A
UNIPROT ID:   P62805 (Gene Accession – BC069654 )
Background:    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Immunogen:    Fusion protein of human H4C1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 219437(H4C1 Antibody) at a dilution of 1/50(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 219437(Anti-H4C1 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 219437(Anti-H4C1 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D227016(Anti-H4C1 Antibody) at dilution 1/50.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: Human cerebella tissue, 293T cell, Mouse spleen tissue, HepG2 cell, Hela cell, Mouse fetal brain tissue, NIH/3T3 cell lysates;
Primary antibody: 219437(H4C1 Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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