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HADH Rabbit pAb

Anti-HADH Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222158

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S222158
Product Name:   Anti-HADH Rabbit Polyclonal Antibody
Synonyms:   HAD; HCDH; HHF4; HADH1; SCHAD; HADHSC; MSCHAD
UNIPROT ID:   Q16836 (Gene Accession – NP_005318 )
Background:    This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15.
Immunogen:    Synthetic peptide of human HADH
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 20-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 222158(HADH Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the synthetic peptide and then with 222158(Anti-HADH Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 222158(Anti-HADH Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with synthetic peptide and then with D264146(Anti-HADH Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: HepG2, 293T and Jurkat cell lysates;
Primary antibody: 222158(HADH Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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