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HAND1 Rabbit pAb

Anti-HAND1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221492

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221492
Product Name:   Anti-HAND1 Rabbit Polyclonal Antibody
Synonyms:   Hxt; eHand; Thing1; bHLHa27
UNIPROT ID:   O96004 (Gene Accession – NP_004812 )
Background:    The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation.
Immunogen:    Synthetic peptide of human HAND1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 221492(HAND1 Antibody) at a dilution of 1/50(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the synthetic peptide and then with 221492(Anti-HAND1 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 221492(Anti-HAND1 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with synthetic peptide and then with D263110(Anti-HAND1 Antibody) at dilution 1/50.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse brain tissue lysate;
Primary antibody: 221492(HAND1 Antibody) at dilution 1/400;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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