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HELT Rabbit pAb

Anti-HELT Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222174

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S222174
Product Name:   Anti-HELT Rabbit Polyclonal Antibody
Synonyms:   Mgn; HESL; HCM1228; bHLHb44
UNIPROT ID:   A6NFD8 (Gene Accession – NP_001287710 )
Background:    Transcriptional repressor which binds preferentially to the canonical E box sequence 5′-CACGCG-3′.
Immunogen:    Synthetic peptide of human HELT
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 20-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 222174(HELT Antibody) at a dilution of 1/30(Nucleus or Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 222174(Anti-HELT Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 222174(Anti-HELT Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with synthetic peptide and then with D264169(Anti-HELT Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Mouse liver tissue, Mouse heart tissue and Mouse brain tissue lysates;
Primary antibody: 222174(HELT Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 minutes
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