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HENMT1 Rabbit pAb

Anti-HENMT1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218679

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218679
Product Name:   Anti-HENMT1 Rabbit Polyclonal Antibody
Synonyms:   HEN1; C1orf59
UNIPROT ID:   Q5T8I9 (Gene Accession – BC012198 )
Background:    Methyltransferase that adds a 2′-O-methyl group at the 3′-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3′-end of piRNAs from uridylation activity and subsequent degradation. Stabilization of piRNAs is essential for gametogenesis.
Immunogen:    Fusion protein of human HENMT1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human kidney cancer tissue using 218679(HENMT1 Antibody) at a dilution of 1/30(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human kidney cancer tissue is first treated with the fusion protein and then with 218679(Anti-HENMT1 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 218679(Anti-HENMT1 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D224933(Anti-HENMT1 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: HEPG2 cell lysate;
Primary antibody: 218679(HENMT1 Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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