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HGD Rabbit pAb

Anti-HGD Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219443

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219443
Product Name:   Anti-HGD Rabbit Polyclonal Antibody
Synonyms:   AKU; HGO
UNIPROT ID:   Q93099 (Gene Accession – BC071757 )
Background:    This gene encodes the enzyme homogentisate 1,2 dioxygenase. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria.
Immunogen:    Fusion protein of human HGD
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 219443(HGD Antibody) at a dilution of 1/90(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 219443(Anti-HGD Antibody) at dilution 1/90.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: A549 and HepG2 cell lysates;
Primary antibody: 219443(HGD Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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