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HIF1AN Rabbit pAb

Anti-HIF1AN Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S214616

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S214616
Product Name:   Anti-HIF1AN Rabbit Polyclonal Antibody
Synonyms:   FIH1
UNIPROT ID:   Q9NWT6 (Gene Accession – NP_060372 )
Background:    Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1alpha. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the beta-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1alpha. This hydroxylation event blocks the association of HIF-1alpha with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1alpha. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm.
Immunogen:    Synthetic peptide of human HIF1AN
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 500-2000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cardiovascular, Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 214616(HIF1AN Antibody) at a dilution of 1/40(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 214616(Anti-HIF1AN Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 214616(Anti-HIF1AN Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D162109(Anti-HIF1AN Antibody) at dilution 1/40.

Gel: 8%SDS-PAGE, Lysate: 60 μg;
Lane 1-2: Mouse brain tissue, Mouse liver tissue;
Primary antibody: 214616(HIF1AN Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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