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HINT3 Rabbit pAb

Anti-HINT3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219848

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219848
Product Name:   Anti-HINT3 Rabbit Polyclonal Antibody
Synonyms:   HINT4
UNIPROT ID:   Q9NQE9 (Gene Accession – NP_612638 )
Background:    Histidine triad proteins, such as HINT3, are nucleotide hydrolases and transferases that act on the alpha-phosphate of ribonucleotides.
Immunogen:    Synthetic peptide of human HINT3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 219848(HINT3 Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 219848(Anti-HINT3 Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 219848(Anti-HINT3 Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with synthetic peptide and then with D260478(Anti-HINT3 Antibody) at dilution 1/60.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: HepG2 cell lysate;
Primary antibody: 219848(HINT3 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 90 seconds
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