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HLA-DMB Rabbit pAb

Anti-HLA-DMB Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222183

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S222183
Product Name:   Anti-HLA-DMB Rabbit Polyclonal Antibody
Synonyms:   RING7; D6S221E
UNIPROT ID:   P28068 (Gene Accession – NP_002109 )
Background:    HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.
Immunogen:    Synthetic peptide of human HLA-DMB
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 222183(HLA-DMB Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the synthetic peptide and then with 222183(Anti-HLA-DMB Antibody) at dilution 1/20.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Raji cell lysate;
Primary antibody: 222183(HLA-DMB Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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