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HLA-DPA1 Rabbit pAb

Anti-HLA-DPA1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S215956

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S215956
Product Name:   Anti-HLA-DPA1 Rabbit Polyclonal Antibody
Synonyms:   PLT1; HLADP; HLASB; DP(W3); DP(W4); HLA-DP1A
UNIPROT ID:   P20036 (Gene Accession – NP_291032 )
Background:    HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules.
Immunogen:    Synthetic peptide of human HLA-DPA1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 40-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 215956(HLA-DPA1 Antibody) at a dilution of 1/35(Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the synthetic peptide and then with 215956(Anti-HLA-DPA1 Antibody) at dilution 1/35.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Raji cell, Human spleen tissue lysates;
Primary antibody: 215956(HLA-DPA1 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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