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HMGCL Rabbit pAb

Anti-HMGCL Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217506

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217506
Product Name:   Anti-HMGCL Rabbit Polyclonal Antibody
Synonyms:   HL
UNIPROT ID:   P35914 (Gene Accession – BC010570 )
Background:    The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human HMGCL
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 217506(HMGCL Antibody) at a dilution of 1/75(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 217506(Anti-HMGCL Antibody) at dilution 1/75.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 217506(Anti-HMGCL Antibody) at a dilution of 1/75.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D222488(Anti-HMGCL Antibody) at dilution 1/75.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Mouse brain tissue, Mouse fetal tissue, Mouse adrenal gland tissue lysates;
Primary antibody: 217506(HMGCL Antibody) at dilution 1/1200;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 second
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