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HSD17B12 Rabbit pAb

Anti-HSD17B12 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217514

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217514
Product Name:   Anti-HSD17B12 Rabbit Polyclonal Antibody
Synonyms:   KAR; SDR12C1
UNIPROT ID:   Q53GQ0 (Gene Accession – BC012043 )
Background:    This gene encodes a very important 17beta-hydroxysteroid dehydrogenase (17beta-HSD) that converts estrone into estradiol in ovarian tissue. This enzyme is also involved in fatty acid elongation. Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in women.
Immunogen:    Fusion protein of human HSD17B12
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Metabolism, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 217514(HSD17B12 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 217514(Anti-HSD17B12 Antibody) at dilution 1/40.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: A172 cells, A375 cells;
Primary antibody: 217514(HSD17B12 Antibody) at dilution 1/650;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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