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HSP90B1 Rabbit pAb

Anti-HSP90B1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216545

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216545
Product Name:   Anti-HSP90B1 Rabbit Polyclonal Antibody
Synonyms:   ECGP; GP96; TRA1; GRP94
UNIPROT ID:   P14625 (Gene Accession – BC009195 )
Background:    This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5′ exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15.
Immunogen:    Fusion protein of human HSP90B1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer, Cardiovascular, Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human brain tissue using 216545(HSP90B1 Antibody) at a dilution of 1/50(Cytoplasm ).

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the fusion protein and then with 216545(Anti-HSP90B1 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 216545(Anti-HSP90B1 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with fusion protein and then with D220724(Anti-HSP90B1 Antibody) at dilution 1/50.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Hela cells, human liver cancer tissue, Jurkat cells, NIH/3T3 cells;
Primary antibody: 216545(HSP90B1 Antibody) at dilution 1/900;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 seconds
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