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HSPA2 Rabbit pAb

Anti-HSPA2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218762

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218762
Product Name:   Anti-HSPA2 Rabbit Polyclonal Antibody
Synonyms:   HSP70-2; HSP70-3
UNIPROT ID:   P54652 (Gene Accession – BC036107 )
Background:    In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Plays a role in spermatogenesis. In association with SHCBP1L may participate in the maintenance of spindle integrity during meiosis in male germ cells.
Immunogen:    Fusion protein of human HSPA2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 218762(HSPA2 Antibody) at a dilution of 1/20(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 218762(Anti-HSPA2 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 218762(Anti-HSPA2 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with fusion protein and then with D225129(Anti-HSPA2 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: 231 cell, Hela cell, Jurkat cell, A549 cell, NIH/3T3 cell, Human cerebrum tissue, Mouse brain tissue lysates;
Primary antibody: 218762(HSPA2 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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