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HSPB1 Rabbit pAb

Anti-HSPB1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221434

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221434
Product Name:   Anti-HSPB1 Rabbit Polyclonal Antibody
Synonyms:   CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
UNIPROT ID:   P04792 (Gene Accession – NP_001531 )
Background:    The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
Immunogen:    Synthetic peptide of human HSPB1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 221434(HSPB1 Antibody) at a dilution of 1/25(Cytoplasm or Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 221434(Anti-HSPB1 Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 221434(Anti-HSPB1 Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with synthetic peptide and then with D263024(Anti-HSPB1 Antibody) at dilution 1/25.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Hepg2, A431, K562, Hela and 293T cell lysates;
Primary antibody: 221434(HSPB1 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 seconds
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