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HSPB6 Rabbit pAb

Anti-HSPB6 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220614

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220614
Product Name:   Anti-HSPB6 Rabbit Polyclonal Antibody
Synonyms:   HEL55; Hsp20; PPP1R91
UNIPROT ID:   O14558 (Gene Accession – NP_653218 )
Background:    The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent poly-peptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The low molecular weight family includes HSP 10, HSP 20, HSP 27 (Heme Oxygenase 1), HSP 32 and HSP 40. HSP 20 occurs in two complex sizes, dimers and multimers. It is related to stress proteins and occurs most abundantly in skeletal muscle and heart. HSP 20 is considerably shorter at the C-terminus and less polar than other small heat shock proteins.
Immunogen:    Synthetic peptide of human HSPB6
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220614(HSPB6 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220614(Anti-HSPB6 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 220614(Anti-HSPB6 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with synthetic peptide and then with D261767(Anti-HSPB6 Antibody) at dilution 1/40.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Rat heart tissue and Human heart tissue lysates;
Primary antibody: 220614(HSPB6 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes
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