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IFITM1 Rabbit pAb

Anti-IFITM1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221377

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221377
Product Name:   Anti-IFITM1 Rabbit Polyclonal Antibody
Synonyms:   9-27; CD225; IFI17; LEU13; DSPA2a
UNIPROT ID:   P13164 (Gene Accession – NP_003632 )
Background:    The IFITM family of proteins are transmembrane proteins so named because their expression is IFN-inducible. IFITM proteins have been found upregulated in human colorectal carcinomas. Both mouse IFITM1 (also known as CD225) and IFITM3 demonstrate expression on the cell surfaces of primordial germ cells in a developmentally-regulated manner. They presumably modulate cell adhesion and influence cell differentiation. IFITM1 activity is required for primordial germ cell transit, and IFITM1 acts as a repulsive molecule by repelling non-IFITM1-expressing primordial germ cells from the mesoderm into the endoderm.
Immunogen:    Synthetic peptide of human IFITM1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 221377(IFITM1 Antibody) at a dilution of 1/20(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 221377(Anti-IFITM1 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 221377(Anti-IFITM1 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D262939(Anti-IFITM1 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela and NIH/3T3 cell lysates;
Primary antibody: 221377(IFITM1 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 seconds
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