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IL18 Rabbit pAb

Anti-IL18 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216578

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216578
Product Name:   Anti-IL18 Rabbit Polyclonal Antibody
Synonyms:   IGIF; IL-18; IL-1g; IL1F4
UNIPROT ID:   Q14116 (Gene Accession – BC007007 )
Background:    The protein encoded by this gene is a proinflammatory cytokine of the IL-1 family that is constitutively found as a precursor within the cytoplasm of a variety of cells including macrophages and keratinocytes. The inactive IL-18 precursor is processed to its active form by caspase-1, and is capable of stimulating interferon gamma production, and of regulating both T helper (Th) 1 and Th2 responses. This cytokine has been implicated in the injury of different organs, and in potentially fatal conditions characterized by a cytokine storm. In humans, IL-18 gene is located on chromosome 11. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human IL18
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cardiovascular, Cancer, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 216578(IL18 Antibody) at a dilution of 1/40(Secreted).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 216578(Anti-IL18 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 216578(Anti-IL18 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D220831(Anti-IL18 Antibody) at dilution 1/40.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela and HepG2 cell lysates;
Primary antibody: 216578(IL18 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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