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IL2RB Rabbit pAb

Anti-IL2RB Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217250

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217250
Product Name:   Anti-IL2RB Rabbit Polyclonal Antibody
Synonyms:   CD122; IMD63; IL15RB; P70-75
UNIPROT ID:   P14784 (Gene Accession – BC025691 )
Background:    The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The protein encoded by this gene represents the beta subunit and is a type I membrane protein. The use of alternative promoters results in multiple transcript variants encoding the same protein. The protein is primarily expressed in the hematopoietic system. The use by some variants of an alternate promoter in an upstream long terminal repeat (LTR) results in placenta-specific expression.
Immunogen:    Fusion protein of human IL2RB
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Immunology, Stem Cells
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 217250(IL2RB Antibody) at a dilution of 1/95(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 217250(Anti-IL2RB Antibody) at dilution 1/95.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: HepG2, 231, Raji, 293T cell lysates;
Primary antibody: 217250(IL2RB Antibody) at dilution 1/700;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 90 seconds
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