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IL6 Rabbit pAb

Anti-IL6 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216576

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216576
Product Name:   Anti-IL6 Rabbit Polyclonal Antibody
Synonyms:   CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2
UNIPROT ID:   P05231 (Gene Accession – BC015511 )
Background:    This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2).
Immunogen:    Fusion protein of human IL6
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cardiovascular,Cancer,Metabolism,Neuroscience,Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216576(IL6 Antibody) at a dilution of 1/50(Secreted).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216576(Anti-IL6 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 216576(Anti-IL6 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with fusion protein and then with D220828(Anti-IL6 Antibody) at dilution 1/50.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: HUVEC, K562, Jurkat, Raji, 231 cell lysates;
Primary antibody: 216576(IL6 Antibody) at dilution 1/400;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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