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KBTBD8 Rabbit pAb

Anti-KBTBD8 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219462

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219462
Product Name:   Anti-KBTBD8 Rabbit Polyclonal Antibody
Synonyms:   TAKRP; TA-KRP
UNIPROT ID:   Q8NFY9 (Gene Accession – BC117487 )
Background:    Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of neural crest specification. The BCR(KBTBD8) complex acts by mediating monoubiquitination of NOLC1 and TCOF1: monoubiquitination promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.
Immunogen:    Fusion protein of human KBTBD8
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 150-300;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 219462(KBTBD8 Antibody) at a dilution of 1/160(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 219462(Anti-KBTBD8 Antibody) at dilution 1/160.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: NIH/3T3 cell lysate;
Primary antibody: 219462(KBTBD8 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 seconds
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