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KDM4A Rabbit pAb

Anti-KDM4A Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S214426

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S214426
Product Name:   Anti-KDM4A Rabbit Polyclonal Antibody
Synonyms:   JMJD2; JHDM3A; JMJD2A; TDRD14A
UNIPROT ID:   O75164 (Gene Accession – NP_055478 )
Background:    This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.
Immunogen:    Synthetic peptide of human KDM4A
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 214426(KDM4A Antibody) at a dilution of 1/40(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 214426(Anti-KDM4A Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 214426(Anti-KDM4A Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D161809(Anti-KDM4A Antibody) at dilution 1/40.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: NIH/3T3 cells, mouse liver tissue;
Primary antibody: 214426(KDM4A Antibody) at dilution 1/650;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 minutes
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