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KRT19 Rabbit pAb

Anti-KRT19 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216317

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216317
Product Name:   Anti-KRT19 Rabbit Polyclonal Antibody
Synonyms:   K19; CK19; K1CS
UNIPROT ID:   P08727 (Gene Accession – BC007628 )
Background:    The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.
Immunogen:    Fusion protein of human KRT19
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 216317(KRT19 Antibody) at a dilution of 1/15(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 216317(Anti-KRT19 Antibody) at dilution 1/15.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 216317(Anti-KRT19 Antibody) at a dilution of 1/15.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D220193(Anti-KRT19 Antibody) at dilution 1/15.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela cells, lovo cells;
Primary antibody: 216317(KRT19 Antibody) at dilution 1/1000;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 50 seconds
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