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KRT2 Rabbit pAb

Anti-KRT2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S210136

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S210136
Product Name:   Anti-KRT2 Rabbit Polyclonal Antibody
Synonyms:   K2e, KRTE, CK-2e, KRT2A, KRT2E
UNIPROT ID:   P35908 (Gene Accession – BC096294 )
Background:    The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Immunogen:    Fusion protein of human KRT2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 210136(KRT2 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 210136(Anti-KRT2 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 210136(Anti-KRT2 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with fusion protein and then with D120237(Anti-KRT2 Antibody) at dilution 1/20.

Gel: 10%SDS-PAGE, Lysate: 30 μg;
Lane: Jurkat cells;
Primary antibody: 210136(KRT2 Antibody) at dilution 1/450;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 45 seconds
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