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LRWD1 Rabbit pAb

Anti-LRWD1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219511

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219511
Product Name:   Anti-LRWD1 Rabbit Polyclonal Antibody
Synonyms:   ORCA; CENP-33
UNIPROT ID:   Q9UFC0 (Gene Accession – BC030547 )
Background:    The protein encoded by this gene interacts with components of the origin recognition complex (ORC) and regulates the formation of the prereplicative complex. The encoded protein stabilizes the ORC and therefore aids in DNA replication. This protein is required for the G1/S phase transition of the cell cycle. In addition, the encoded protein binds to trimethylated histone H3 in heterochromatin and recruits the ORC and lysine methyltransferases, which help maintain the repressive heterochromatic state. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human LRWD1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Developmental Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 219511(LRWD1 Antibody) at a dilution of 1/80(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 219511(Anti-LRWD1 Antibody) at dilution 1/80.

The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 219511(Anti-LRWD1 Antibody) at a dilution of 1/80.

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with fusion protein and then with D227442(Anti-LRWD1 Antibody) at dilution 1/80.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: PC-3 cell lysate;
Primary antibody: 219511(LRWD1 Antibody) at dilution 1/300;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 minutes
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