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MAN1B1 Rabbit pAb

Anti-MAN1B1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218908

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218908
Product Name:   Anti-MAN1B1 Rabbit Polyclonal Antibody
Synonyms:   MRT15; ERMAN1; ERManI; MANA-ER
UNIPROT ID:   Q9UKM7 (Gene Accession – BC002953 )
Background:    This gene encodes an enzyme belonging to the glycosyl hydrolase 47 family. This enzyme functions in N-glycan biosynthesis, and is a class I alpha-1,2-mannosidase that specifically converts Man9GlcNAc to Man8GlcNAc isomer B. It is required for N-glycan trimming to Man5-6GlcNAc2 in the endoplasmic-reticulum-associated degradation pathway. Mutations in this gene cause autosomal-recessive intellectual disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 11.
Immunogen:    Fusion protein of human MAN1B1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 218908(MAN1B1 Antibody) at a dilution of 1/145(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 218908(Anti-MAN1B1 Antibody) at dilution 1/145.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: HL-60 cell lysate;
Primary antibody: 218908(MAN1B1 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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