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MAP2K3 Rabbit pAb

Anti-MAP2K3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216701

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216701
Product Name:   Anti-MAP2K3 Rabbit Polyclonal Antibody
Synonyms:   MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
UNIPROT ID:   P46734 (Gene Accession – BC032478 )
Background:    The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.
Immunogen:    Fusion protein of human MAP2K3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Epigenetics and Nuclear Signaling, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 216701(MAP2K3 Antibody) at a dilution of 1/80(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 216701(Anti-MAP2K3 Antibody) at dilution 1/80.

The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 216701(Anti-MAP2K3 Antibody) at a dilution of 1/80.

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with fusion protein and then with D221082(Anti-MAP2K3 Antibody) at dilution 1/80.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela and Raji cell lysates;
Primary antibody: 216701(MAP2K3 Antibody) at dilution 1/550;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
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