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MAP3K11 Rabbit pAb

Anti-MAP3K11 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220716

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220716
Product Name:   Anti-MAP3K11 Rabbit Polyclonal Antibody
Synonyms:   MLK3; PTK1; SPRK; MLK-3; MEKK11
UNIPROT ID:   Q16584 (Gene Accession – NP_002410 )
Background:    The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42.
Immunogen:    Synthetic peptide of human MAP3K11
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220716(MAP3K11 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220716(Anti-MAP3K11 Antibody) at dilution 1/50.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: K562, NIH/3T3, PC3, LO2, MCF7 cell lysates;
Primary antibody: 220716(MAP3K11 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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