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MAPK4 Rabbit pAb

Anti-MAPK4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219768

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219768
Product Name:   Anti-MAPK4 Rabbit Polyclonal Antibody
Synonyms:   ERK4; ERK-4; PRKM4; p63MAPK; p63-MAPK
UNIPROT ID:   P31152 (Gene Accession – NP_002738 )
Background:    Mitogen-activated protein kinase 4 is a member of the mitogen-activated protein kinase family. Tyrosine kinase growth factor receptors activate mitogen-activated protein kinases which then translocate into the nucleus and phosphorylate nuclear targets. Alternative splicing results in multiple transcript variants.
Immunogen:    Synthetic peptide of human MAPK4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 219768(MAPK4 Antibody) at a dilution of 1/35(Cytoplasm or Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 219768(Anti-MAPK4 Antibody) at dilution 1/35.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 219768(Anti-MAPK4 Antibody) at a dilution of 1/35.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with synthetic peptide and then with D260319(Anti-MAPK4 Antibody) at dilution 1/35.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: 293T cell, Human heart tissue, Raji and LNCAP cell lysates;
Primary antibody: 219768(MAPK4 Antibody) at dilution 1/550;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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