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MAPKAPK2 Rabbit pAb

Anti-MAPKAPK2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220695

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220695
Product Name:   Anti-MAPKAPK2 Rabbit Polyclonal Antibody
Synonyms:   MK2; MK-2; MAPKAP-K2
UNIPROT ID:   P49137 (Gene Accession – NP_116584 )
Background:    This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.
Immunogen:    Synthetic peptide of human MAPKAPK2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220695(MAPKAPK2 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220695(Anti-MAPKAPK2 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 220695(Anti-MAPKAPK2 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D261888(Anti-MAPKAPK2 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: A549 cells;
Primary antibody: 220695(MAPKAPK2 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 minutes
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