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MARCKSL1 Rabbit pAb

Anti-MARCKSL1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219245

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219245
Product Name:   Anti-MARCKSL1 Rabbit Polyclonal Antibody
Synonyms:   F52; MLP; MRP; MLP1; MACMARCKS
UNIPROT ID:   P49006 (Gene Accession – BC007904 )
Background:    This gene encodes a member of the myristoylated alanine-rich C-kinase substrate (MARCKS) family. Members of this family play a role in cytoskeletal regulation, protein kinase C signaling and calmodulin signaling. The encoded protein affects the formation of adherens junction. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are located on the long arm of chromosomes 6 and 10.
Immunogen:    Fusion protein of human MARCKSL1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 219245(MARCKSL1 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the fusion protein and then with 219245(Anti-MARCKSL1 Antibody) at dilution 1/50.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: RAW264.7 cell lysate;
Primary antibody: 219245(MARCKSL1 Antibody) at dilution 1/750;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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