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MCAM Rabbit pAb

Anti-MCAM Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S213885

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S213885
Product Name:   Anti-MCAM Rabbit Polyclonal Antibody
Synonyms:   CD146; MUC18; HEMCAM; METCAM; MelCAM
UNIPROT ID:   P43121 (Gene Accession – NP_006491 )
Background:    Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Immunogen:    Synthetic peptide of human MCAM
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 40-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cardiovascular, Immunology, Stem Cells
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 213885(MCAM Antibody) at a dilution of 1/40(Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 213885(Anti-MCAM Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 213885(Anti-MCAM Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D161008(Anti-MCAM Antibody) at dilution 1/40.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: Human leiomyosarcoma tissue lysate;
Primary antibody: 213885(MCAM Antibody) at dilution 1/400;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 seconds
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