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MCM3 Rabbit pAb

Anti-MCM3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216643

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216643
Product Name:   Anti-MCM3 Rabbit Polyclonal Antibody
Synonyms:   HCC5, P1.h, RLFB, P1-MCM3
UNIPROT ID:   P25205 (Gene Accession – BC001626 )
Background:    The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human MCM3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216643(MCM3 Antibody) at a dilution of 1/30(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216643(Anti-MCM3 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 216643(Anti-MCM3 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with fusion protein and then with D220966(Anti-MCM3 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: K562 cells, Jurkat cells, hela cells;
Primary antibody: 216643(MCM3 Antibody) at dilution 1/550;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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