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MCM6 Rabbit pAb

Anti-MCM6 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216639

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216639
Product Name:   Anti-MCM6 Rabbit Polyclonal Antibody
Synonyms:   Mis5; P105MCM; MCG40308
UNIPROT ID:   Q14566 (Gene Accession – BC032374 )
Background:    The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication.
Immunogen:    Fusion protein of human MCM6
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 216639(MCM6 Antibody) at a dilution of 1/60(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 216639(Anti-MCM6 Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 216639(Anti-MCM6 Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D220961(Anti-MCM6 Antibody) at dilution 1/60.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: 293T cells, A549 cells, Raji cells, K562 cells, NIH/3T3 cells;
Primary antibody: 216639(MCM6 Antibody) at dilution 1/433.3;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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