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MED28 Rabbit pAb

Anti-MED28 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217939

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217939
Product Name:   Anti-MED28 Rabbit Polyclonal Antibody
Synonyms:   EG1; magicin; 1500003D12Rik
UNIPROT ID:   Q9H204 (Gene Accession – BC011936 )
Background:    Med28 (mediator complex subunit 28), also known as EG1 (endothelial-derived protein 1), magicin (merlin and Grb2-interacting cytoskeletal protein) or tumor angiogenesis marker EG-1, is a 178 amino acid subunit of the Mediator complex that localizes to both nucleus and cytoplasm.
Immunogen:    Full length fusion protein
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 217939(MED28 Antibody) at a dilution of 1/30(Cytoplasm or Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 217939(Anti-MED28 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 217939(Anti-MED28 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D223403(Anti-MED28 Antibody) at dilution 1/30.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Human placenta tissue;
Primary antibody: 217939(MED28 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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