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MED30 Rabbit pAb

Anti-MED30 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219231

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219231
Product Name:   Anti-MED30 Rabbit Polyclonal Antibody
Synonyms:   MED30S; THRAP6; TRAP25
UNIPROT ID:   Q96HR3 (Gene Accession – BC008226 )
Background:    The multiprotein TRAP/Mediator complex facilitates gene expression through a wide variety of transcriptional activators. MED30 is a component of this complex that appears to be metazoan specific (Baek et al., 2002 [PubMed 11909976]).
Immunogen:    Fusion protein of human MED30
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 219231(MED30 Antibody) at a dilution of 1/80(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 219231(Anti-MED30 Antibody) at dilution 1/80.

The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 219231(Anti-MED30 Antibody) at a dilution of 1/80.

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with fusion protein and then with D226099(Anti-MED30 Antibody) at dilution 1/80.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane: RAW264.7 cell lysate;
Primary antibody: 219231(MED30 Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 seconds
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