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MED6 Rabbit pAb

Anti-MED6 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217943

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217943
Product Name:   Anti-MED6 Rabbit Polyclonal Antibody
Synonyms:   ARC33; NY-REN-28
UNIPROT ID:   O75586 (Gene Accession – BC004106 )
Background:    Mediator of RNA polymerase II transcription subunit 6 is one of the subunits of the Mediator complex. It is an enzyme that in humans is encoded by the MED6 gene. Component of the Mediator comple,x a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.
Immunogen:    Full length fusion protein
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human brain tissue using 217943(MED6 Antibody) at a dilution of 1/30(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the fusion protein and then with 217943(Anti-MED6 Antibody) at dilution 1/30.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Hela and PC3 cell, 231 cell and human esophagus cancer tissue;
Primary antibody: 217943(MED6 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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