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MOB1A Rabbit pAb

Anti-MOB1A Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220720

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220720
Product Name:   Anti-MOB1A Rabbit Polyclonal Antibody
Synonyms:   MOB1; MATS1; Mob4B; C2orf6; MOBK1B; MOBKL1B
UNIPROT ID:   Q9H8S9 (Gene Accession – NP_060691 )
Background:    MOB1 was first identified in yeast as a protein that binds to Mps with essential roles in the completion of mitosis and the maintenance of ploidy. Its Drosophila and mammalian homologs, Mats and MOB1, respectively, are involved in the Hippo signaling tumor suppressor pathway, which plays a critical role in organ size regulation and which has been implicated in cancer development . There are two MOB1 proteins in humans, MOB1à and MOB1?, that are encoded by two different genes but which have greater than 95% amino acid sequence identity.
Immunogen:    Synthetic peptide of human MOB1A
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220720(MOB1A Antibody) at a dilution of 1/30(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220720(Anti-MOB1A Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 220720(Anti-MOB1A Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D261918(Anti-MOB1A Antibody) at dilution 1/30.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Hela cells, Jurkat cells, mouse liver tissue, K562 cells, A375 cells;
Primary antibody: 220720(MOB1A Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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