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MYL12B Rabbit pAb

Anti-MYL12B Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221530

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221530
Product Name:   Anti-MYL12B Rabbit Polyclonal Antibody
Synonyms:   MLC-B; MRLC2
UNIPROT ID:   O14950 (Gene Accession – NP_291024 )
Background:    The activity of nonmuscle myosin II is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments.
Immunogen:    Synthetic peptide of human MYL12B
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 221530(MYL12B Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 221530(Anti-MYL12B Antibody) at dilution 1/25.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Human urinary bladder tissue, Mouse urinary bladder tissue lysates;
Primary antibody: 221530(MYL12B Antibody) at dilution 1/500;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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