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NAIF1 Rabbit pAb

Anti-NAIF1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216670

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216670
Product Name:   Anti-NAIF1 Rabbit Polyclonal Antibody
Synonyms:   C9orf90; bA379C10.2
UNIPROT ID:   Q69YI7 (Gene Accession – BC021580 )
Background:    Human NAIF1 is located on chromosome 9q34.11 and encodes 327 amino acids with a homeodomain-like region and two nuclear localization signals at its N-terminal region. NAIF1 is conserved across diverse species, including human, mouse, crab-eating macaque, dog, chicken and frog, and shares no obvious homology to any known genes or proteins. NAIF1 was predominantly localized in the nucleus. Overexpression of NAIF1 inhibited cell growth and induced apoptosis. Furthermore, NAIF1 transfection caused both decreases in mitochondrial membrane potential and caspase-3 activation. In summary, NAIF1 is a nuclear protein that induces apoptosis when overexpressed.
Immunogen:    Fusion protein of human NAIF1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 216670(NAIF1 Antibody) at a dilution of 1/100(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 216670(Anti-NAIF1 Antibody) at dilution 1/100.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: C2C12 cell lysate;
Primary antibody: 216670(NAIF1 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
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