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NCBP1 Rabbit pAb

Anti-NCBP1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S215561

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S215561
Product Name:   Anti-NCBP1 Rabbit Polyclonal Antibody
Synonyms:   NCBP; Sto1; CBP80
UNIPROT ID:   Q09161 (Gene Accession – NP_002477 )
Background:    The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5′ cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3′-end processing, RNA nuclear export, and nonsense-mediated mRNA decay.
Immunogen:    Synthetic peptide of human NCBP1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 215561(NCBP1 Antibody) at a dilution of 1/20(Nucleus or Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the synthetic peptide and then with 215561(Anti-NCBP1 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 215561(Anti-NCBP1 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D163556(Anti-NCBP1 Antibody) at dilution 1/20.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: Jurkat, NIH/3T3, 293T, Hela, HT-29 and HEPG2 cell lysates;
Primary antibody: 215561(NCBP1 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds
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