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NCK1 Rabbit pAb

Anti-NCK1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216672

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216672
Product Name:   Anti-NCK1 Rabbit Polyclonal Antibody
Synonyms:   NCK, nck-1, NCKalpha
UNIPROT ID:   P16333 (Gene Accession – BC006403 )
Background:    The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
Immunogen:    Fusion protein of human NCK1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: Oct-50;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 216672(NCK1 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 216672(Anti-NCK1 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 216672(Anti-NCK1 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D221022(Anti-NCK1 Antibody) at dilution 1/20.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane: Hela cells;
Primary antibody: 216672(NCK1 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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