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NDUFA8 Rabbit pAb

Anti-NDUFA8 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220736

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220736
Product Name:   Anti-NDUFA8 Rabbit Polyclonal Antibody
Synonyms:   PGIV; CI-19KD; CI-PGIV
UNIPROT ID:   P51970 (Gene Accession – NP_055037 )
Background:    The protein encoded by this gene belongs to the complex I 19 kDA subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays an important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Immunogen:    Synthetic peptide of human NDUFA8
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 220736(NDUFA8 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the synthetic peptide and then with 220736(Anti-NDUFA8 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 220736(Anti-NDUFA8 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D261941(Anti-NDUFA8 Antibody) at dilution 1/40.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane: 293T cells;
Primary antibody: 220736(NDUFA8 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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