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NDUFAF4 Rabbit pAb

Anti-NDUFAF4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220738

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220738
Product Name:   Anti-NDUFAF4 Rabbit Polyclonal Antibody
Synonyms:   My013; C6orf66; HRPAP20; HSPC125; bA22L21.1
UNIPROT ID:   Q9P032 (Gene Accession – NP_054884 )
Background:    NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene are a cause of mitochondrial complex I deficiency.
Immunogen:    Synthetic peptide of human NDUFAF4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 220738(NDUFAF4 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the synthetic peptide and then with 220738(Anti-NDUFAF4 Antibody) at dilution 1/50.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Jurkat cells, Hela cells, K562 cells;
Primary antibody: 220738(NDUFAF4 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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