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NDUFB3 Rabbit pAb

Anti-NDUFB3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218059

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218059
Product Name:   Anti-NDUFB3 Rabbit Polyclonal Antibody
Synonyms:   B12; CI-B12
UNIPROT ID:   O43676 (Gene Accession – BC018183 )
Background:    This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which is the first enzyme in the electron transport chain of mitochondria. This protein localizes to the inner membrane of the mitochondrion as a single-pass membrane protein. Mutations in this gene contribute to mitochondrial complex 1 deficiency.
Immunogen:    Full length fusion protein
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 218059(NDUFB3 Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 218059(Anti-NDUFB3 Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 218059(Anti-NDUFB3 Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with fusion protein and then with D223632(Anti-NDUFB3 Antibody) at dilution 1/25.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: PC3 and hela cell;
Primary antibody: 218059(NDUFB3 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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